Ikrimah narrates from Ibn Abbas (may Allah be pleased with them both) that the Prophet (peace and blessings be upon him) said: "Make use of ithmid (kohl), for it sharpens the eyesight and makes the hair grow." He stated that the Messenger of Allah (peace and blessings be upon him) had a kohl container from which he (peace and blessings be upon him) applied kohl every night, three times in each eye.
This is the most authentic of what has been narrated about the Prophet (peace and blessings be upon him) applying kohl.
Hadith Referenceالسنن الكبرى للبيهقي / كتاب الصيام / 8257